However, Ander Herrera, who took part in training this week, did not make the squad.
Martial has not played for the Red Devils since the 2-0 defeat to Paris Saint-Germain on February 12, while Matic has been sidelined since the 2-0 win over Chelsea in the FA Cup on February 18.
Mason Greenwood, who made his senior debut as a late substitute in Paris, also keeps his place in the squad alongside Tahith Chong.
Manchester United Squad:
GOALKEEPERS: David De Gea, Sergio Romero, Lee Grant
DEFENDERS: Chris Smalling, Victor Lindelof, Eric Bailly, Luke Shaw, Diogo Dalot, Ashley Young, Marcos Rojo
MIDFIELDERS: Paul Pogba, Nemanja Matic, Fred, Andreas Pereira, Scott McTominay, Tahith Chong
FORWARDS: Marcus Rashford, Romelu Lukaku, Anthony Martial, Mason Greenwood